Phosphorylation and activation of human cdc25-C by cdc2--cyclin B and its involvement in the self-amplification of MPF at mitosis.
作者： E. Karsenti , G. Draetta , I. Hoffmann , P.R. Clarke , M.J. Marcote
DOI: 10.1002/J.1460-2075.1993.TB05631.X
关键词:
摘要: We have investigated the mechanisms responsible for sudden activation of cdc2-cyclin B protein kinase before mitosis. It has been found previously that cdc25 is tyrosine phosphatase dephosphorylating and activating B. In Xenopus eggs early embryos a homologue undergoes periodic phosphorylation activation. Here we show catalytic activity human cdc25-C also activated directly by in mitotic cells. Phosphorylation HeLa extracts or increases its activity. not substrate cyclin A-associated kinases. able to activate B1 egg induce oocyte maturation, but only after stable thiophosphorylation. This demonstrates required entry into M-phase. Together, these studies offer plausible explanation rapid at onset mitosis self-amplification MPF observed vivo.
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