Cyanogen bromide cleavage and proteolytic peptide mapping of proteins immobilized to membranes.

摘要: Publisher Summary This chapter discusses the cyanogen bromide cleavage and proteolytic peptide mapping of proteins immobilized to membranes. Peptide is widely used for study sites protein phosphorylation. In case that must be purified by gel electrophoresis, samples are eluted from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide then precipitated with trichloroacetic acid (TCA) in presence carrier proteins. However, this procedure sample preparation can laborious tedious result considerable loss radioactive material. contrast protocol digestion proteins, it very important oxidation done after tryptic No peptides immobilon-P membrane trypsin if carried out first. It likely strong makes binding irreversible.