SELECTIVE SOLID-PHASE EXTRACTION OF CATECHOLAMINES AND METANEPHRINES FROM SERUM USING A NEW MOLECULARLY IMPRINTED POLYMER

摘要: A new commercially molecularly imprinted polymer dedicated to the specific solid phase extraction of catecholamines and metanephrines has been tested for neurotransmitters in serum samples. protocol was developed especially several aminergic metabolites (dopamine, 3-methoxytyramine, epinephrine, norepinephrine, normetanephrine, serotonine). The diluted water (1/10, v/v), loaded on a cartridge filled with washed successively water, methanol-water (15/85, methanol. Analytes were then eluted methanol-acetic acid (99/1, v/v). No matrix peak observed chromatograms obtained by high performance liquid chromatography coupled ultra-violet detection (281 nm). High selectivities between nonimprinted polymers. Extraction recoveries ranged from 64–98% (RSD <6%, n = 4) depending type analytes linearity assessed 0.30–0.90 µM concentratio...