Analysis of flavonoid content, antioxidant, antimicrobial and antibiofilm activity of in vitro hairy root extract of radish ( Raphanus sativus L.)

摘要: The production of biofilm inhibiting antimicrobial agents plant origin is gaining significant importance in recent years. inhibition by natural considered safe and provides greater scope for alternative strategies. hairy root culture extracts established infecting leaf explants with Agrobacterium rhizogenes R1000 A4 strains Raphanus raphanistrum L. subsp. sativus (L.) Domin (syn. R. L.) have been studied this regard. total flavonoids phenolics contents were estimated their influence on the antioxidant antibiofilm activities investigated. strain produced 90.0% roots while 81.0% Murashige Skoog (hormone-free ½ MS) medium containing 75 µM acetosyringone within three days. hormone-free MS 116.8 mM sucrose concentration highest biomass accumulation (14.11 g/L Fresh Weight (FW); 1.78 g/L Dry (DW); Growth Index (GI) 7.06) after 30 days compared to MS, B5 (Gamborg B5) media. integration T-DNA Ri plasmid was confirmed Polymerase Chain Reaction (PCR) analysis based amplification rolB rolC genes. induced extract (RHRE) high amount (52.0 mg/g DW) (67.0 mg/g DW). quercetin content RHRE (488.4 mg/g determined RP-HPLC LC–MS/MS. exhibited 92% against stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical ferric reducing power (FRAP) assay absorbance 3.62 at 593 nm. also activity Gram-negative Gram-positive bacteria, particularly MDR bacterium Pseudomonas aeruginosa, formation EPS bacterium. Our findings indicate industrial applications as potent agent probably due enriched content. could be used prevent food contamination spoilage environment. Factors affecting optimized Raphnus (L.). displayed better results A4. Optimum flavonoid obtained ½-strength 4% sucrose. higher antimicrobial, activity. present study a novel approach improve can food industry.