Molecular and functional characterization of the murine ldh2 promoter region: Sp-binding GC-box domains are the key cis-elements regulating ldh2 gene expression during spermatogenesis

摘要: The goal of the present study was to elucidate specific transcriptional mechanisms that regulate ldh2 gene expression during early stages spermatogenesis. DNA sequence analysis 1.0-kb promoter region directly upstream start site indicated presence three SP-protein binding GC-box elements and absence TATA CAAT boxes. Functional characterization studies mouse were performed in SV40 transformed spermatogonial cell line, GC-1 spg. Transfection/transient using full-length truncated promoter/luciferase reporter constructs revealed all SP-binding cis-regulatory are required for optimal activity. Additional site-directed mutagenesis two most proximal sites play essential regulatory roles mediating basal These suggest spermatogonia spermatocytes regulated by SP-mediated mechanisms.