Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy
作者： L.M. Carlin , C. Auffray , F. Geissmann
DOI: 10.1002/0471142735.IM1433S101
关键词:
摘要: This unit describes methods for intravital imaging of monocytes in the vasculature dermis and mesentery vivo using fluorescent reporter mice, dyes, antibodies. Cx3cr1gfp/gfp (or +), Rag2−/−, Il2rg−/− mice expressing eGFP at locus Cx3cr1 gene, on Rag2−/− C57Bl/6 background, are used. Although aimed specifically tracking Ly6Clow monocytes, these protocols could readily be adapted to investigate interaction other blood leukocytes with vascular endothelium by use fluorescently labeled Curr. Protoc. Immunol. 101:14.33.1-14.33.16. © 2013 John Wiley & Sons, Inc. Keywords: Ly6Clow monocytes; intravital microscopy; cell tracking; confocal microscopy; blood vessels; in
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