White adipocyte vascular endothelial growth factor: regulation by insulin.

摘要: White adipose tissue from rats was examined for insulin- responsive vascular endothelial growth factor 165 (VEGF) secretion and mRNA expression. When separated into it constituent fat vs. stromal-vascular cells using collagenase digestion methods, only the adipocytes (or whole tissue) responded to physiological insulin concentrations by doubling VEGF release over 4 24 h in culture. Adipocyte expression increased similarly. Several depots were tested. Although omental had highest rates of release, differences not significant. Insulin-stimulated mediated part via PI3K, but PKC. Additional hormones/agents tested, including steroids, leptin, an adenosine analog, norepinephrine. Only latter compound production, this effect adenylate cyclase. Adjusting incubation glucose concentration between 0–20 mm did alter adipocyte release. An experimental mimic hypoxia, CoCl2, also ...