Label-free detection of DNA single-base mismatches using a simple reflectance-based optical technique.

摘要: Rapid and quantitative detection of the binding nucleic acids to surface-immobilized probes remains a challenge in many biomedical applications. We investigated hybridization set fully complementary defected 12-base long DNA oligomers by using Reflective Phantom Interface (RPI), recently developed multiplexed label-free technique. Based on simple measurement reflected light intensity, this technology enables quantify directly as it occurs surface with sensitivity 10 pg mm−2. found strong effect single-base mismatches their location kinetics equilibrium binding. In line previous studies, we that DNA–DNA is weaker than bulk. Our data indicate consequence weak nonspecific surface.