On-line enrichment of N-glycans by immobilized metal-affinity monolith for capillary electrophoresis analysis.
作者： Huikai Shao , Balazs Reider , Gabor Jarvas , Andras Guttman , Zhengjin Jiang
DOI: 10.1016/J.ACA.2020.08.002
关键词:
摘要: Abstract A novel N-glycan enrichment strategy is presented using unexpected but strong interactions between the sulfonate groups brought by fluorescent dye of glycans and Zr4+ modified poly(ethylene glycol methacrylate phosphate (EGMP)-co-acrylamide (AM)-co-bis-acrylamide (BAA)) monolith. The poly (EGMP-co-AM-co-BAA) monolith was synthesized via ultraviolet (UV) irradiation then functionalized with Zr4+. obtained characterized scanning electron microscopy mercury intrusion porosimetry. Large through-pores a continuous skeleton high permeability were observed. N-glycans labeled 1-aminopyrene-3, 6, 8-trisulfonic acid (APTS) enriched through IMAC interaction. This step coupled off-line to capillary electrophoresis (CE) separation laser induced fluorescence (LIF) detection. Successful preconcentration APTS maltooligosaccharide ladder achieved under optimized conditions. Enrichment factors for maltooligosaccharides ranged from 9 24 RSDs 2.0% 9.2% (n = 3). Moreover, very good repeatabilities (
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