Fluorometric assay of secondary amino acids

作者: Manfred Weigele , Silvano DeBernardo , Willy Leimgruber

DOI: 10.1016/0006-291X(73)90847-4

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摘要: Abstract A method is described for the conversion of secondary amino acids to primary amines which can be assayed with fluorescamine (I). Secondary undergo oxidative decar☐ylation when reacted halogenating agents. The resulting imines are hydrolyzed amines, subsequently allowed react (I) yield fluorescent pyrrolinones (II). This reaction sequence provides an efficient fluorometric assay acids. Thus, procedure now applicable full array natural

参考文章(1)
M. Weigele, S. L. DeBernardo, J. P. Tengi, W. Leimgruber, Novel reagent for the fluorometric assay of primary amines Journal of the American Chemical Society. ,vol. 94, pp. 5927- 5928 ,(1972) , 10.1021/JA00771A084