Human Oral Buccal Microbiomes Are Associated with Farmworker Status and Azinphos-Methyl Agricultural Pesticide Exposure.

摘要: In a longitudinal agricultural community cohort sampling of 65 farmworker and 52 non-farmworker adults, we investigated pesticide exposure associated changes in the oral buccal microbiota. We found seasonally persistent association between detected blood concentration insecticide Azinphos-methyl taxonomic composition swab microbiome. Blood samples were collected concurrently from individual subjects two seasons, spring/summer 2005 winter 2006. Mass spectrometry quantified concentrations organophosphate Azinphos-methyl. Buccal microbiome 16S rRNA gene DNA sequenced, assigned to bacterial taxonomy analyzed after ‘centered-log-ratio9 transformation handle compositional nature proportional abundances bacteria per sample. Non-parametric analysis transformed data for individuals with without detection showed significant perturbations seven common genera (>0.5% sample mean read depth), including reductions bacteria, Streptococcus . Diversity individuals9 microbiomes was also using principal components (PCA) reveal primary PCA clusters types. The ‘exposed9 cluster significantly less diversity enriched farmworkers contained 27 30 who had blood. Importance this study show human that is large-scale alterations microbiota extinctions whole suggested some individuals. Persistence suggests long lasting effects on commensal have occurred. important health related outcomes these are not understood at time. Future investigations should index medical dental records chronic diseases may be interactively caused exposure-microbiome alteration association.