OSMOLYTE STRATEGY IN HUMAN MONOCYTES AND MACROPHAGES : INVOLVEMENT OF P38MAPK IN HYPEROSMOTIC INDUCTION OF BETAINE AND MYOINOSITOL TRANSPORTERS
作者： Carsten Denkert , Ulrich Warskulat , Frank Hensel , Dieter Häussinger
关键词:
摘要: Betaine and myoinositol are compatible organic osmolytes which specifically accumulated by cells exposed to hyperosmotic medium. A role for in the regulation of immune function rat liver macrophages has been described recently. This report describes an osmolyte strategy human peripheral blood monocytes blood-derived macrophages. Hyperosmotic (405 mOsm) exposure led upregulation betaine/gamma-amino-n-butyric acid (GABA) transporter BGT-1 sodium-dependent SMIT mRNA levels within 6 12 h. Induction occurred regardless whether hyperosmolarity was induced addition NaCl (50 mM) or raffinose (100 mM). (5 inhibited as well mRNA. After uptake betaine increased up 10-fold compared normoosmotic conditions. Hypoosmotic a rapid efflux myoinositol. Treatment with pyridinyl imidazole SB 203580 (10 microM), specific inhibitor p38 MAP kinase, hyperosmolarity-induced increase 45-70%. The data show that use when osmotic stress p38MAPK is involved transporters SMIT.
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