Cloning of a Na(+)- and Cl(-)-dependent betaine transporter that is regulated by hypertonicity.
作者： A Yamauchi , S Uchida , H M Kwon , A S Preston , R B Robey
DOI: 10.1016/S0021-9258(18)48543-2
关键词:
摘要: Many hypertonic bacteria, plants, marine animals, and the mammalian renal medulla are protected from deleterious effects of high intracellular concentrations electrolytes by accumulating nonperturbing osmolyte betaine. When kidney-derived Madin-Darby canine kidney (MDCK) cells cultured in medium, they accumulate betaine to 1,000 times its medium concentration. This results induction hypertonicity rates transport into cells. We have isolated a cDNA (BGT-1) encoding transporter screening an MDCK cell library for expression Xenopus oocytes. The encodes single protein 614 amino acids, with estimated molecular weight 69 kDa. deduced acid sequence exhibits highly significant topographic similarity brain gamma-amino-n-butyric (GABA) noradrenaline transporters, suggesting that BGT-1 is member GABA/noradrenaline gene family. Expression oocytes indicates has both GABA activities Cl(-)- as well Na(+)-dependent functionally similar Northern hybridization mRNA localized induced hypertonicity.
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sci-hub.st 参考文章(19)
Mary McCormick, [33] Sib selection Methods in Enzymology. ,vol. 151, pp. 445- 449 ,(1987) , 10.1016/S0076-6879(87)51036-9
R.B. Robey, H.M. Kwon, J.S. Handler, A. Garcia-Perez, M.B. Burg, Induction of glycinebetaine uptake into Xenopus oocytes by injection of poly(A)+ RNA from renal cells exposed to high extracellular NaCl. Journal of Biological Chemistry. ,vol. 266, pp. 10400- 10405 ,(1991) , 10.1016/S0021-9258(18)99239-2
S Bagnasco, R Balaban, H M Fales, Y M Yang, M Burg, Predominant osmotically active organic solutes in rat and rabbit renal medullas. Journal of Biological Chemistry. ,vol. 261, pp. 5872- 5877 ,(1986) , 10.1016/S0021-9258(17)38464-8
Jack Kyte, Russell F. Doolittle, A simple method for displaying the hydropathic character of a protein Journal of Molecular Biology. ,vol. 157, pp. 105- 132 ,(1982) , 10.1016/0022-2836(82)90515-0
Tadeusz Pacholczyk, Randy D. Blakely, Susan G. Amara, Expression cloning of a cocaine- and antidepressant-sensitive human noradrenaline transporter. Nature. ,vol. 350, pp. 350- 354 ,(1991) , 10.1038/350350A0
J.W. Ferkany, L.A. Smith, W.E. Seifert, R.M. Caprioli, S.J. Enna, Measurement of gamma-aminobutyric acid (GABA) in blood Life Sciences. ,vol. 22, pp. 2121- 2128 ,(1978) , 10.1016/0024-3205(78)90456-3
P. Yancey, M. Clark, S. Hand, R. Bowlus, G. Somero, Living with water stress: evolution of osmolyte systems Science. ,vol. 217, pp. 1214- 1222 ,(1982) , 10.1126/SCIENCE.7112124
Lindsey Needham, Nicholas J.F. Dodd, Miles D. Houslay, Quinidine and melittin both decrease the fluidity of liver plasma membranes and both inhibit hormone-stimulated adenylate cyclase activity. Biochimica et Biophysica Acta. ,vol. 899, pp. 44- 50 ,(1987) , 10.1016/0005-2736(87)90237-9
JOHN C. MEASURES, Role of amino acids in osmoregulation of non-halophilic bacteria Nature. ,vol. 257, pp. 398- 400 ,(1975) , 10.1038/257398A0
Ernest M. Wright, Richard E. Schell, Bruce R. Stevens, Specificity of intestinal brush-border proline transport: cyanine dye studies. Biochimica et Biophysica Acta. ,vol. 818, pp. 271- 274 ,(1985) , 10.1016/0005-2736(85)90568-1