Practical detection for simultaneous analysis of multiple antigens with Staphylococcal protein A as an intermediate

摘要: Simultaneous measurements of multiple protein biomarkers are typically required to avoid false results in clinical diagnosis. In this work, we present an immunochemical event between antibody and antigen for simultaneous analysis double biomarkers. the presence Staphylococcal A (SPA), two kinds antibodies were captured on Ni–NTA agarose beads a site-oriented model. Two target antigens fluorescence-labeled then added turn form immunosorbent complex (antibody–antigen–antibody). The standard sandwich immunoassay can be visualized using fluorescence microscope detect respective bound bead's surface. Meanwhile, freshly prepared gold-coated substrate was modified with 3-aminopropyl triethoxysilane (APTES) through glutaraldehyde linker. SPA–antibody–antigen also validated quartz crystal microbalance (QCM) apparatus. showed appreciable increase frequency shift when SPA, antibody, onto chip, respectively. Qualitative synchronous quantitative QCM biosensors practical methods detection serological