Amplified signal in binding assays
作者： Edwin F. Ullman , Rajendra Singh , Steve de Keczer , Dariush Davalian
DOI:
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摘要: Methods are disclosed for determining minute quantities of an analyte in a medium suspected containing the analyte. One method comprises treating under conditions such that analyte, if present, causes substrate having oxidant cleavable linker and photosensitizer to come into close proximity. The generates singlet oxygen which oxidatively cleaves form product can be detected sandwich detection assay as LOCI. amount is related medium. Compositions, kits, compounds also disclosed.
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