Simultaneous determination of reduced and oxidized ubiquinones.

摘要: Publisher Summary Quantitative analyses of individual ubiquinones (Q) homologs in biological samples have been performed by high-performance liquid chromatography (HPLC) combined with an ultraviolet spectrometric detector (UVD) or mass spectrometry (MS). An electrochemical (ECD) for HPLC was confirmed to be simple and sensitive the determination Q. However, only Q determined these methods. For QH 2 (ubiquinol) mitochondria, submitochondrial particle cell-free bacterial homogenates, dual-wavelength method has generally used. The method, however, cannot simultaneously measure amounts whole tissues owing presence vitamin A other interfering compounds, which absorbance same spectral region as undergo absorption change chemical reduction. separately determine homologs. analytical procedure described developed provide a rapid, sensitive, direct assay samples. This is based on extraction from tissues, microsomal fractions, plasma organic solvents, followed quantitation means reversed-phase UVD ECD.