Response to acute hCG stimulation and steroidogenic potential of Leydig cell fibroblastic precursors in humans.

摘要: The process of early testosterone (T) secretion and Leydig cell differentiation in humans was studied to explore the steroidogenic capacity fibroblastic precursors. Seven cryptorchid boys received hCG prior orchidopexy. Patients CP, PB, MR one injection 1000 IU; patients JR GG, three daily injections IU, MP MM, five IU. A testicular biopsy obtained at time operation, 24 hours after last injection. Serum T (ng/dl) before stimulation (ng/g) were determined by RIA. control prepubertal testis (tumoral orchidectomy) incubated vitro showed a time-dependent accumulation both medium tissue. Testosterone released into 1, 2, 4 0.76, 1.43, 4.03 ng/ml, respectively. Tissue 0, 9, 11, 16, ng/g, This indicates synthesis medium. Control testes abundant precursors with scanty cytoplasm, few organelles, heterochromatic nuclei, minute nucleoli. No cells present. After 1 day stimulation, numerous fibroblasts activated, displaying enlarged cytoplasms increased numbers nuclei rich euchromatin, bigger Basal serum 58.2 +/- 45.3 ng/dl 87.3 42.0 administration, while 974.0 686.0 ng/g (control is 10-50 ng/g). 3 days hCG, activated immature appeared. 35.5 7.8 394.0 24.0 rose 2797.5 1222.6 ng/g. 5 days, mature appeared for first time. 58 59.3 (basal) 641.5 390 (after hCG); 789 (patient MM did not have value T). HCG induced coated pits endocytic vesicles young cells, suggesting receptor aggregation internalization hormone-receptor complexes. Peroxidase-antiperoxidase (PAP) localization positive peritubular cells.(ABSTRACT TRUNCATED AT 400 WORDS)