A Non-Tumorigenic Human Liver Epithelial Cell Culture Model for Chemical and Biological Carcinogenesis Investigations

摘要: A new medium has been formulated that will support in vitro replication of normal human liver epithelial cells for 4 passages (12 population doublings). The replicating uniformly contain keratin 18, but 19 is not detectable. In addition, albumin remains discernable many the throughout their culture life-span. SV40 large T-antigen gene introduced into genome from six cultures were initiated both adult and neonatal donors. life spans two these are indefinite whereas, others have extended doubling potentials. transformed near-normal karyotypes and, initially, they express cytokeratin albumin, cytochromes. With continued passaging, however, became positive also loose expression most hepato-specific proteins. However, when incubated as roller cultures, re-acquire ability to metabolize benzo[a]pyrene, aflatoxin B1, dimethylnitrosamine electrophilic derivatives form carcinogen-DNA adducts. Further, co-cultured with fibroblasts or on extracted basement membrane material, synthesize acute phase proteins.Therefore, by using appropriate conditions, “immortalized” may serve a useful model chemical biological carcinogenesis studies.