THE EFFECTS OF CARBON MONOXIDE INHIBITION ON ATP LEVEL AND THE RATE OF MITOSIS IN THE SEA URCHIN EGG.

作者: David Epel

DOI: 10.1083/JCB.17.2.315

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摘要: The requirements for ATP synthesis during the various phases of mitosis were investigated in oxygen-requiring eggs sea urchin, Strongylocentrotus purpuratus. CO dark, a specific inhibitor respiration, was used to inhibit synthesis. kinetics respiratory inhibition determined by analyzing levels with luciferin-luciferase assay. mitotic analysis rate mitosis. It found that resulted decrease normal level. Coincident this which stops completely when drops below 50 per cent With use degrees inhibition, is shown be related resultant These results contradict basic premise energy reservoir hypothesis, and also disagree other reports cells are insensitive inhibitors metabolism. Data presented demonstrate these conflicting result from insufficient above findings all indicate depends on continuous utilization ATP.

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