Cell counts in the human cerebral cortex using a traditional and an automatic method

摘要: This study compares the results of cell counting in human cerebral cortex by a traditional and an automatic procedure. Four blocks were taken from standard areas each 10 brains individuals aged 18-95 years showing no neurological abnormality. Sections block counted methods, both which are described. The method used photomicrographs procedure quantimet 720 Image Analysing Computer. degree reproducibility was measured discussed tabulated. Comparison made total counts, counts cells measuring greater than 20 um across. show high level correlation between two procedures but manual count yields higher number for smaller numbers large method. reasons these discrepancies examples arterfacts produce them giver. Using described amount meterial examined took about 4 months to study. sane tissue can be assessed 1 day using apparatus. speed close achieved makes it valuable aid quantitative studies central nervous system.