Identification of strain diversity and phylogenetic analysis based on two major essential proteins of Orf viruses isolated from several clinical cases reported in Malaysia.

摘要: There is a little information on the characterization of Orf virus strains that are endemic in Malaysia. The relationship between severity disease and molecular genetic profile has not been fully elucidated. This study documented first confirmed report contagious ecthyma causing by goats from selected state eastern peninsular causes significant debilitation due to inability affected animals suckle which brings great economic loss farmers. A total 504 were examined individually recognize with lesion. Skin scrapping was used collect scab material infected animals. presence combination methods including isolation vero cells, identification Transmission Electron Microscopy (TEM) technique using PCR Sanger sequencing. results showed successful four typical cytopathic effects cultured cells line. morphology be distinctive ovoid criss cross structure. phylogenetic analysis revealed these isolated closely related each other previously Malaysian orf viruses. In addition viruses China India. provides more valuable insight terms genotype circulating