High-frequency transformation of the fission yeast Schizosaccharomyces pombe

摘要: The fission yeast, Schizosaccharomyces pombe, has been used extensively for genetic studies but until now it not utilized as a host organism DNA cloning. Here we describe method high-frequency transformation of leu 1− strain this yeast with hybrid plasmids containing the Saccharomyces cerevisiae LEU 2+ gene, bacterial plasmid and either S. 2 µm or autonomously replicating sequences (ars)1 derived from pombe DNA. Some contain unique restriction sites which make them suitable isolation genes, they can also be exchange between cerevisiae.