The isolation of protoplasts of the fission yeast Schizosaccharomyces by Trichoderma viride and snail enzymes.

摘要: The formation of protoplasts the fission yeastsSchizosaccharomyces pombe andSchizosaccharomyces versatilis after combined application snail enzymes andTrichoderma viride in an osmotic stabilizer (0.4m KC1, pH 5.5) was studied by light and electron microscopy. effect used leads during 30 min to 100% protoplast population. Using microscopy no original walls or wall remnants were detected suspension protoplasts. Protoplasts are viable liquid nutrient medium they regenerate cell revert into normal cells. Such a population may be useful for biochemical study metabolism quantitative methods as well chemical regenerating walls.