Orthophosphate determination: Interference by mannitol and sorbitol

作者: Chong H. Ho , Shri V. Pande

DOI: 10.1016/0003-2697(74)90249-8

关键词:

摘要: Abstract Mannitol and sorbitol interfered with the orthophosphate estimation based on formation of phosphomolybdate complex. Binding molybdate was cause interference as increasing concentration ammonium eliminated interference. Thus, for in presence mannitol, latter must be kept at below inhibitory level.

参考文章(10)
Grant R. Bartlett, Phosphorus Assay in Column Chromatography Journal of Biological Chemistry. ,vol. 234, pp. 466- 468 ,(1959) , 10.1016/S0021-9258(18)70226-3
Jorg W. Stucki, Paul Walter, Pyruvate Metabolism in Mitochondria from Rat Liver FEBS Journal. ,vol. 30, pp. 60- 72 ,(1972) , 10.1111/J.1432-1033.1972.TB02072.X
William E. Jacobus, Albert L. Lehninger, Creatine Kinase of Rat Heart Mitochondria Journal of Biological Chemistry. ,vol. 248, pp. 4803- 4810 ,(1973) , 10.1016/S0021-9258(19)43737-X
R. Huxtable, R. Bressler, Determination of orthophosphate Analytical Biochemistry. ,vol. 54, pp. 604- 608 ,(1973) , 10.1016/0003-2697(73)90393-X
R. Parvin, Roberts A. Smith, Determination of inorganic phosphate in the presence of labile organic phosphates. Analytical Biochemistry. ,vol. 27, pp. 65- 72 ,(1969) , 10.1016/0003-2697(69)90219-X
C. van der Heiden, J. Desplanque, On the determination of orthophosphate as a phosphomolybdate-vanadate complex Analytical Biochemistry. ,vol. 43, pp. 622- 628 ,(1971) , 10.1016/0003-2697(71)90296-X
Cyrus H. Fiske, Yellapragada Subbarow, THE COLORIMETRIC DETERMINATION OF PHOSPHORUS Journal of Biological Chemistry. ,vol. 66, pp. 375- 400 ,(1925) , 10.1016/S0021-9258(18)84756-1
Nelson K. Richtmyer, C. S. Hudson, The Rotation of Polyols in Ammonium Molybdate Solutions Journal of the American Chemical Society. ,vol. 73, pp. 2249- 2250 ,(1951) , 10.1021/JA01149A095
Isaac Berenblum, Ernst Chain, Studies on the colorimetric determination of phosphate Biochemical Journal. ,vol. 32, pp. 286- 294 ,(1938) , 10.1042/BJ0320286