Use of flow cytometry to compare the antimicrobial efficacy of silver-containing wound dressings against planktonic Staphylococcus aureus and Pseudomonas aeruginosa.

摘要: Silver-impregnated wound dressings continue to be routinely used for the management of infected wounds, or wounds that are at risk becoming infected. The ability antimicrobials have been incorporated into kill microorganisms within dressing requires appropriate evaluation using in vitro models. In models exploited this purpose included corrected zone inhibition and log reduction assay. However, these other related culturable-based assays purported poor correlation with overall microbicidal barrier activity an antimicrobial dressing. This is because methods only retrospectively indicate bacterial cell death do not take account viable but nonculturable states microorganisms. Consequently, it was study show use flow cytometry, conjunction Syto(®) 9 propidium iodide, could as a method accurately evaluating comparing efficacy silver alginate carboxymethyl cellulose on individual cells without need culturable assays. When comparison planktonic Staphylococcus aureus simulated fluid assay made between each dressing, enhanced (as showed by percentage dead alive cells) shown. Pseudomonas aeruginosa exposed both silver-containing dressings, equivalent rates were up 4 days. result significantly different (p<0.05). By utilizing cytometric assays, can evaluated enabling differentiation achieved live bacteria. considered significant advancement traditionally presently testing