Electrochemical Nitric Oxide Microsensors Based on a Fluorinated Xerogel Screening Layer for in Vivo Brain Monitoring.

摘要: Nitric oxide (NO) is an important free radical synthesized and released by brain cells. At low (nanomolar) levels, it modulates synaptic transmission neuronal activity, but at much higher levels mediates injury through oxidative stress. However, the precise concentrations which these biological actions are exerted still poorly defined. Electrochemical detection of NO in vivo requires rigorous exclusion endogenous redox molecules such as ascorbate or nitrite. A fluorinated xerogel composed trimethoxymethylsilane heptadecafluoro-1,1,2,2-tetrahydrodecyl silane has been proposed to create a screening layer around sensors, protecting against chemical interference vitro. Here we detected living using carbon fiber microelectrodes covered with nickel porphyrin this xerogel. These microsensors were insensitive interfering surpassed similar coated Nafion layer. In vivo, rat parietal cortex, electrodes could detect local microinjection glutamatergic agonist N-methyl-d-aspartate (NMDA). NMDA-evoked release peaked 1.1 μM lasted more than 20 min. This can therefore be applied allowing for fabrication highly specific study physio-pathological brain.