Development and evaluation of an indirect enzyme immunoassay for detection of porcine antibodies to pseudorabies virus.

摘要: An indirect enzyme immunoassay is described for detection of porcine serum antibody to pseudorabies virus. The analytical sensitivity the was found be approximately 4.5 log 4 X 10 (5120 times) greater than neutralization test, based on parallel end point titrations. diagnostic comparable or superior that neutralization, earliest detectable after infection swine with virus by intranasal intrauterine routes contact infected pigs. immunoassay, at a screening dilution 1:20, gave 100% agreement ELISA results provided U.S. Department Agriculture-Animal and Plant Health Inspection Service proficiency panel 40 sera. One having demonstrable seronegative test.