The effect of bovicin HC5, a bacteriocin from Streptococcus bovis HC5, on ruminal methane production in vitro.

摘要: Methane represents a loss of feed energy to ruminant animals, and nutritionists have sought methods inhibiting ruminal methane production. When mixed bacteria (approximately 400 mg protein ml(-1)) from cow fed timothy hay were incubated in vitro with carbon dioxide hydrogen (0.5 atm) for less than 8 h, the first-order rate production was 17 micromol ml(-1). Semi-purified bacteriocin Streptococcus bovis HC5 (bovicin HC5) inhibited production, by as much 50%, even low concentration bovicin (128 activity units (AU) caused significant decrease. Mixed that transferred successively retained their ability produce hydrogen, did not Cultures treated AU gradually lost methane, detected after four transfers. These latter results indicated methanogens could adapt become resistant HC5. chromosomal DNA amplified 16S rDNA primers specific archaea, digested restriction enzymes (HhaI HaeIII) separated on agarose gels, approximately 12 fragments observed. control cultures (third transfer) had same fragment pattern indicating selective. Given perception routine use antibiotics animal feeds should be avoided, bacteriocins may provide an alternative strategy decreasing