A nude mouse model of hypertrophic scar shows morphologic and histologic characteristics of human hypertrophic scar.

摘要: Hypertrophic scar (HSc) is a fibroproliferative disorder that occurs following deep dermal injury. Lack of relevant animal model one barrier toward better understanding its pathophysiology. Our objective to demonstrate grafting split-thickness human skin onto nude mice results in survival engrafted and murine scars are morphologically, histologically, immunohistochemically consistent with HSc. Twenty were xenografted skin. Animals euthanized at 30, 60, 120, 180 days postoperatively. Eighteen controls autografted full-thickness mouse 30 60 Scar biopsies harvested each time point. Blinded assessment was performed using modified Manchester Scale. Histologic analysis included hematoxylin eosin, Masson's trichrome, toluidine blue, picrosirius red staining. Immunohistochemistry anti-human leukocyte antigen-ABC, α-smooth muscle actin, decorin, biglycan Xenografted developed red, shiny, elevated similar HSc supported by blinded assessment. Autograft appeared morphologically histologically normal Xenografts survived up showed increased thickness, loss hair follicles, adnexal structures rete pegs, hypercellularity, whorled collagen fibers parallel the surface, myofibroblasts, decreased decorin expression, mast cell density. Grafting persistent show morphologic, histologic, immunohistochemical consistency Therefore, this provides promising technique study formation test novel treatment options.