[4] Molecular genetics of Escherichia coli leader peptidase
作者： Takaya Sudate , Pamela Silver , William Wickner
DOI: 10.1016/0076-6879(83)97117-3
关键词:
摘要: Publisher Summary This chapter describes the methods for identification of leader peptidase gene and genetic manipulations to determine role in cell growth. Leader is identified detergent extracts whole cells by its insensitivity serine protease inhibitors ability posttranslationally cleave M13 procoat coat protein. It important understand normal functionings cell. Biochemical analysis has indicated that purified process several membrane protein precursors their native size vitro . The liposomes reconstituted from Escherichia coli phospholipids not only correctly protein, but also mediate correct integration into lipid bilayer. These findings suggest a critical
sci-hub.se 参考文章(11)
C. Zwizinski, W. Wickner, Purification and characterization of leader (signal) peptidase from Escherichia coli. Journal of Biological Chemistry. ,vol. 255, pp. 7973- 7977 ,(1980) , 10.1016/S0021-9258(19)43928-8
David Botstein, Ronald W. Davis, John R. Roth, Advanced bacterial genetics Cold Spring Harbor Laboratory. ,(1980)
C. Zwizinski, T. Date, W. Wickner, Leader peptidase is found in both the inner and outer membranes of Escherichia coli. Journal of Biological Chemistry. ,vol. 256, pp. 3593- 3597 ,(1981) , 10.1016/S0021-9258(19)69649-3
P B Wolfe, P Silver, W Wickner, The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme. Journal of Biological Chemistry. ,vol. 257, pp. 7898- 7902 ,(1982) , 10.1016/S0021-9258(18)34466-1
Jeffrey H Miller, Experiments in molecular genetics ,(1972)
M. Monk, J. Kinross, Conditional Lethality of recA and recB Derivatives of a Strain of Escherichia coli K-12 with a Temperature-Sensitive Deoxyribonucleic Acid Polymerase I Journal of Bacteriology. ,vol. 109, pp. 971- 978 ,(1972) , 10.1128/JB.109.3.971-978.1972
Joe Hedgpeth, Marc Ballivet, Harvey Eisen, Lambda phage promoter used to enhance expression of a plasmid-cloned gene. Molecular Genetics and Genomics. ,vol. 163, pp. 197- 203 ,(1978) , 10.1007/BF00267410
David T. Kingsbury, Donald R. Helinski, DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1 Biochemical and Biophysical Research Communications. ,vol. 41, pp. 1538- 1544 ,(1970) , 10.1016/0006-291X(70)90562-0
W. Wickner, T. Killick, Membrane-associated assembly of M13 phage in extracts of virus-infected Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 505- 509 ,(1977) , 10.1073/PNAS.74.2.505
Louise Clarke, John Carbon, A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome Cell. ,vol. 9, pp. 91- 99 ,(1976) , 10.1016/0092-8674(76)90055-6