Neovascularization of synthetic membranes directed by membrane microarchitecture

摘要: Transplantation of tissues enclosed within a membrane device designed to protect the cells from immune rejection (immunoisolation) provides an opportunity treat variety disease conditions. Successful implementation immunoisolation has been hampered by foreign-body reaction biomaterials. We screened commercially available membranes for reactions following implantation under skin rats. Histologic analysis revealed that neovascularization at membrane-tissue interface occurred in several had pore sizes large enough allow complete penetration host (0.8-8 microns size). When vascularization 5-microns-pore-size polytetrafluoroethylene (PTFE) was compared 0.02-microns-pore-size PTFE membranes, it found larger 80-100-fold more vascular structures. The increased observed even though laminated smaller inner prevent cell entry into prototype device. This significantly higher level maintained 1 year subcutaneous site