Site-specific cleavage of basement membrane collagen IV during Drosophila metamorphosis
作者： Liselotte I. Fessler , John H. Fessler , Maureen L. Condic , Robert E. Nelson , James W. Fristrom
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摘要: Breakdown of basement membranes is an important step in the controlled rearrangement cells during metamorphosis, cell migration, and metastatic spread tumor cells. One our two laboratories found a unique collagenous peptide that only appears metamorphosis Drosophila melanogaster. The other laboratory previously reported 20-hydroxyecdysone-induced eversion imaginal discs glycoprotein named gp125 arises (Birr et al., 1990). We show these peptides are identical they formed from membrane collagen IV. Cleavage occurs at imperfection this homotrimeric helix between residues 755/756 sequence CALDE/IKMPAK. carboxyl fragment, 100,647 M(r), as derived amino acid alpha 1(IV) chain. corresponding fragment was also recovered disulfide-linked aggregate. This specific cleavage supports concept highly targeted, breakdown metamorphosis. Furthermore, cuts occur strategic sites predicted supramolecular network IV molecules membranes.
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