Rice black-streaked dwarf virus P10 suppresses protein kinase C in insect vector through changing the subcellular localization of LsRACK1.

摘要: Rice black-streaked dwarf virus (RBSDV) was known to be transmitted by the small brown planthopper (SBPH) in a persistent, circulative and propagative manner nature. Here, we show that RBSDV major outer capsid protein (also as P10) suppresses kinase C (PKC) activity of SBPH through interacting with receptor for activated 1 (LsRACK1). The N terminal P10 (amino acids (aa) 1-270) LsRACK1 (aa 268-315) were mapped crucial interaction. Confocal microscopy subcellular fractionation showed fused enhanced green fluorescent formed vesicular structures associated endoplasmic reticulum (ER) membranes Spodoptera frugiperda nine cells. Our results also indicated retargeted initial localization from cytoplasm cell membrane ER affected function LsRACKs activate PKC. Inhibition RACK1 double stranded RNA-induced gene silencing significantly promoted replication SBPH. In addition, PKC pathway participates antivirus innate immune response This study highlights negatively regulates accumulation activating signalling pathway, changes affects its article is part theme issue 'Biotic sheds light on smart pest management'.