Temporal and spatial control of cyclin B1 destruction in metaphase.
作者： Paul Clute , Jonathon Pines
DOI: 10.1038/10049
关键词:
摘要: The proteolysis of key regulatory proteins is thought to control progress through mitosis. Here we analyse cyclin B1 degradation in real time and find that it begins as soon the last chromosome aligns on metaphase plate, just after spindle-assembly checkpoint inactivated. At this point, staining disappears from spindle poles chromosomes. Cyclin destruction can subsequently be inactivated throughout if reimposed, correlates with reappearance These results provide a temporal spatial link between ubiquitin-mediated proteolysis.
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