Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models.

摘要: Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are Mycoplasma mycoides subsp. Mycoides (Mmm), the aetiological agent of contagious bovine pleuropneumonia and capri (Mmc), which causes pneumonia, mastitis, arthritis, keratitis, septicemia goats. We established precision cut lung slices (PCLS) infection model for Mmm Mmc study host-pathogen interactions. monitored over time using immunohistological analysis electron microscopy. Moreover, burden was plating quantitative real-time PCR. Results were compared with lungs from experimentally infected goats cattle. Lungs healthy cattle also included as controls. PCLS remained viable up two weeks. Both subspecies adhered ciliated cells. However, titer caprine decreased time, indicating specificity Mmm. showed higher tropism sub-bronchiolar tissue PCLS, increased a time-dependent manner. abundantly observed on pulmonary endothelial cells, partially, how it systemic disease. Tissue destruction upon prolonged comparable vivo samples. Therefore, represents novel ex interaction livestock mycoplasma.