Proteomic characterization of pleural effusion, a specific host niche of Mycoplasma mycoides subsp. mycoides from cattle with contagious bovine pleuropneumonia (CBPP)

摘要: Mycoplasma mycoides subsp. (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a severe in cattle. The abnormal accumulation pleural fluid, called effusion (PE), one characteristics this disease. We performed proteomic analysis seven PE samples from experimentally infected cattle and characterized their composition with respect to Mmm proteins. detected total 963 different Further indicated strong enrichment proteins involved antigen processing, platelet activation degranulation apoptosis an increased abundance acute phase proteins.With regard pathogen, up 108 viable mycoplasma cells per ml were supernatant. revealed 350 proteins, including virulence-associated processes like hydrogen peroxide (H2O2) production capsule synthesis. will aid characterize inflammasome during identified serve as baseline data be compared vitro studies improve our understanding pathogenicity mechanisms. Based on results, we named “in vivo niche” CBPP. Biological significance: This first study effusions derived infectious disease approach proteome vivo. high number effusion. infection qualitatively similar plasma, but differs On other hand,Mmm its natural host produces synthesis, H2O2 induction inflammatory response, supporting previous knowledge mechanisms underlying survival virulence pathogen while inside host. forms profound basis for testing protein candidates diagnostics or vaccines.