The characterization, molecular cloning, and expression of a novel hematopoietic cell antigen from CD34+ human bone marrow cells

摘要: The adhesion molecule BEN/SC1/DM-GRASP (BEN) is a marker in the developing chicken nervous system that also expressed on surface of embryonic and adult hematopoietic cells such as immature thymocytes, myeloid progenitors, erythroid progenitors. F84.1 KG-CAM, two monoclonal antibodies to rat neuronal glycoproteins with similarity BEN, cross-react an antigen but only recognizes human blood cell We have defined recognized by (HCA). HCA expression was detected 40% 70% CD34 + fetal bone marrow mobilized peripheral cells. Precursor activity for long-term vitro culture confined subset coexpress HCA. most primitive subsets cells, including all rhodamine 123 lo , Thy-1 CD38 −/lo progenitors not early B-cell describe here cloning characterization cDNAs encoding variants (huHCA-1 huHCA-2) cDNA clone (raHCA). deduced amino acid sequences huHCA raHCA are homologous BEN. Recombinant proteins produced from either or were F84.1, whereas antirat KG-CAM. Expression form CHO conferred homophilic could be competed soluble recombinant huHCA-Fc. molecular availability should permit further evaluation its role rodent hematopoiesis.