Protein HU binds specifically to kinked DNA
作者： Andrea Pontiggia , Armando Negri , Monica Beltrame , Marco E. Bianchi
DOI: 10.1111/J.1365-2958.1993.TB01126.X
关键词:
摘要: We have purified the main four-way junction DNA-binding protein of Escherichia coli, and found it to be well-known HU protein. recognizes with high-affinity one angles present in junction, a molecule shape an X. Other DNA structures characterized by sharp bends or kinks, like bulged duplex DNAs containing unpaired bases, are also bound. appears inhibit cruciform extrusion from supercoiled inverted repeat (palindromic) DNA, either constraining supercoiling trapping metastable interconversion intermediate. All these properties analogous mammalian chromatin HMG1. suggest that is prokaryotic HMG1-like rather than histone-like
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