Physical factors determining the early local tissue reactions produced by food colourings and other compounds injected subcutaneously

摘要: Abstract A study has been made of the physicochemical basis early local tissue reaction elicited by repeated subcutaneous injections water-soluble food colourings in rat. With exception Erythrosine BS, were all absorbed equally rapidly and uniformly, whether from undamaged or grossly fibrosed tissue. Hence retention at injection site could not account for observed differences investigated. The properties found to play a significant part determining surface activity, lipid solubility protein-binding ability, particularly when latter was associated with alteration protein structure. No evidence be aggregation colouring its ability interact calf-thymus DNA vitro . course erythrocyte haemolysis disintegration cholesterol-lecithin mixed micelles under influence increasing concentrations sodium salts Patent Blue V VRS appeared identical corresponding effects brought about surfactants lauryl sulphate triton X-100. Other triphenylmethane possessing considerable activity Light Green SF Yellowish, Fast FCF Brilliant FCF. Most xanthene tested possessed high tricaprylin micelles. While displaying these affected integrity membranes very low concentrations, manner closely similar that amphipathic compounds saponin lysolecithin. capacity bind and, so doing, alter structure resided FCF, BS Indigo Carmine. None described present any appreciable extent calcium VRS, Violamine R azo studied. physical correlate type it. In presence marked degree solubility, extensive necrosis resulted initially determined development IV reaction. Binding with, of, modified changes provoking macrophage response leading III exceptional binding considerably diminished lipophilic property, consequent reduction damage. Colourings none attributes produced mild effect (type I reaction) while self-limiting II) if only minimal degree. Our investigations lead conclusion ultimately, formation sarcomas some studied can fully accounted on characteristics compounds.