MiR-22 is frequently downregulated in medulloblastomas and inhibits cell proliferation via the novel target PAPST1.

摘要: Medulloblastoma is the most frequent malignant central nervous system tumor in children. MicroRNAs (miRs) are small, non-coding RNAs that target protein-coding and RNAs, play roles a variety of cellular processes through regulation multiple targets. In present study, we analyzed miR-22 expression its effect cell proliferation apoptosis medulloblastomas. Quantitative reverse transcription PCR (RT-PCR) revealed significantly lower 19 out 27 (70%) medulloblastomas, D341, DAOY, ONS-76 medulloblastoma lines, compared with normal cerebellum. Forced by lentiviral vector transfection reduced induced apoptosis, while knockdown increased proliferative activity DAOY cells. cells overexpression nude mice yielded tumors smaller than those originated from control Microarray analysis forced showed significant changes profiles, PAPST1 being (10 folds) downregulated gene. RT-PCR PAPST1 mRNA upregulation 18 (67%) addition, luciferase reporter assay suggested directly targets gene, lentivirus-mediated suppressed These results suggest frequently associated this may be at least part via PAPST1, which novel miR-22.