Rewiring host activities for synthetic circuit production: a translation view.

摘要: The expression of synthetic circuits in host organisms, or chassis, is a key aspect biology. Design adjustments made for maximal production may negatively affect the central metabolism and biosynthetic activities chassis host. Here, we review recent attempts to modulate circuit design optimal present models that precisely capture trade-off between growth. We also emerging concepts full orthogonalization productivity its decoupling from endogenous cell, opening new routes towards robust expression.