Optimization of Translation Profiles Enhances Protein Expression and Solubility

摘要: mRNA is translated with a non-uniform speed that actively coordinates co-translational folding of protein domains. Using structure-based homology we identified the structural domains in epoxide hydrolases (EHs) and introduced slow-translating codons to delineate translation single These changes dramatically improved solubility two EHs metagenomic origin Escherichia coli. Conversely, importance transient attenuation for folding, consequently solubility, EH was evidenced member family from Agrobacterium radiobacter, which partitions soluble fraction when expressed E. Synonymous substitutions shaping slow-transiting regions fast-translating render this insoluble. Furthermore, show low yield can be enhanced by decreasing free energy initial 5’-coding region, disrupt secondary structure enhance ribosomal loading. This study provides direct experimental evidence not mere messenger into amino acids but bears an additional layer information expression level encoded protein. it general frame on how modulate fine-tune gene target