An Integrated Approach Reveals Regulatory Controls on Bacterial Translation Elongation

摘要: Ribosomes elongate at a nonuniform rate during translation. Theoretical models and experiments disagree on the in vivo determinants of elongation mechanism by which affects protein levels. To resolve this conflict, we measured transcriptome-wide ribosome occupancy under multiple conditions used it to formulate whole-cell model translation in E. coli. Our predicts that rates most codons nutrient-rich growth are not limited intracellular concentrations aminoacyl-tRNAs. However, pausing starvation for single amino acids is highly sensitive kinetics tRNA aminoacylation. We further show abortion upon accounts observed along mRNAs starvation. Abortion reduces global synthesis, but enhances subset mRNAs. These results suggest regulatory role aminoacylation stress, our study provides an experimentally constrained framework modeling