Widespread Co-translational RNA Decay Reveals Ribosome Dynamics

摘要: Summary It is generally assumed that mRNAs undergoing translation are protected from decay. Here, we show are, in fact, co-translationally degraded. This a widespread and conserved process affecting most genes, where 5′–3′ transcript degradation follows the last translating ribosome, producing an in vivo ribosomal footprint. By sequencing ends of 5′ phosphorylated mRNA degradation intermediates, obtain genome-wide drug-free measurement ribosome dynamics. We identify general termination pauses both normal stress conditions. In addition, describe novel codon-specific pausing sites response to oxidative dependent on RNase Rny1. Our approach simple straightforward does not require use translational inhibitors or in vitro RNA footprinting can alter protection patterns.