Maintenance of Mouse Nephron Progenitor Cells in Aggregates with Gamma-Secretase Inhibitor
作者： Shunsuke Yuri , Masaki Nishikawa , Naomi Yanagawa , Oak D. Jo , Norimoto Yanagawa
DOI: 10.1371/JOURNAL.PONE.0129242
关键词:
摘要: Knowledge on how to maintain and expand nephron progenitor cells (NPC) in vitro is important provide a potentially valuable source for kidney replacement therapies. In our present study, we examined the possibility of optimizing NPC maintenance "re-aggregate" system. We found that Six2-expressing (Six2+)-NPC could be maintained aggregates reconstituted with dispersed from E12.5 mouse embryonic kidneys at least up 21 days culture. The Six2+-NPC required presence ureteric bud cells. number increased by more than 20-fold day 21, but plateaued after 14. an attempt further sustain proliferation passage subculture, new (P1) original (P0) failed NPC. However, based similarity between P1 derived E15.5 kidneys, suspected differentiated may interfere maintenance. support this notion, preventing differentiation DAPT, γ-secretase inhibitor inhibits Notch signaling pathway, was effective 65-fold. retained their potential epithelialize upon exposure Wnt signal. conclusion, demonstrated study "re-aggregation" system can useful when combined inhibitor.
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