Comparison of the non-specific binding of unfractionated heparin and low molecular weight heparin (Enoxaparin) to plasma proteins

摘要: The non-specific binding of anticoagulantly-active heparin to plasma proteins may influence its anticoagulant effect. We used low affinity (LAH) essentially devoid anti-factor Xa activity investigate the extent and possible mechanism this binding. addition excess LAH platelet-poor containing a fixed amount unfractionated doubled presumably because it displaces from proteins. Although dextran sulfates varying molecular weights also increased activity, less sulfated heparin-like polysaccharides had no These findings suggest that ability displace active protein sites is related charge be independent size. In contrast effect in heparin, there was little augmentation when added weight (LMWH), indicating LMWH binds than heparin. This concept supported by studies comparing with buffer antithrombin III. 2-fold purified system. contrast, identical both buffer, respectively. clinically relevant recovered 33% lower patients suspected venous thrombosis healthy volunteers.(ABSTRACT TRUNCATED AT 250 WORDS)