Virome analysis of antiretroviral-treated HIV patients shows no correlation between T-cell activation and anelloviruses levels

摘要: Abstract Background Abnormally high levels of T-cell activation can persist in HIV-infected subjects despite effective anti-retroviral therapy (ART) and has been associated with negative health outcomes. The nature the antigenic drivers or other causes this residual remain uncertain. Anelloviruses are universally acquired soon after birth, resulting persistent viremia, considered part commensal human virome. Reduced immunocompetence results increased anellovirus levels. Objectives To test whether anelloviruses viruses plasma higher during ART. Study design Two amplification methods combined next generation sequencing were used to detect all estimate relative from 19 adults on ART who exhibited a wide range Results Nucleic acids HBV HCV detected one patient each while pegivirus A (GBV-C) was found three patients. Anellovirus DNA patients some individuals carrying up eight different genotypes. Specific genotypes level co-infections not activation. No association between percentage activated CD4 CD8 T cells. Conclusions Human HIV suppressed subjects, viremia levels, genetically highly diverse. correlated present indicating that antigens unlikely be dominant source driving chronic