A New Cytokine-Dependent Monoblastic Cell Line With t(9;11)(p22;q23) Differentiates to Macrophages With Macrophage Colony-Stimulating Factor (M-CSF) and to Osteoclast-Like Cells With M-CSF and Interleukin-4

摘要: Monocytes/macrophages exert a series of important functions in vivo. To facilitate detailed investigation their functional capacity and the mechanism leading to differentiation, several cell lines have been established from primary material. We present here new human monoblastic line, designated UG3. UG3 cells are characterized by following features. (1) harbor t(9;11)(p22;q23) translocation that results fusion MLL AF9 genes produce corresponding AF9-MLL MLL-AF9 transcripts. (2) rely on presence exogenous growth factors for viability proliferation, such as interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte (G-CSF), or macrophage (M-CSF). (3) When cultured G-CSF, differentiate along granulocytic lineage, evidenced segmentation nuclei positive staining neutrophilic alkaline phosphatase peroxidase. (4) GM-CSF M-CSF, into mature macrophages while preserving surface expression CD14 CD68 also start release cytokines cell-culture supernatants. Under these culture conditions, take up acetylated LDL. (5) M-CSF IL-4, osteoclast-like multinucleated giant capable bone resorption display tartrate-resistant acid (TRAP) activity. thus provide features qualify them useful model further investigate underlying processes elucidate role monocytes/macrophages osteoclasts.