MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma

摘要: // Shupeng Sun 1, * , Xiuyu Wang 2, Xinnv Xu 3, Hui Di 4 Jixiang Du 2 Bin Qiong 1 Jinhuan Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Neurosurgical Institute, Department Neurosurgery, Huanhu Hospital, 300350, China The Graduate School, Medical University, 300070, 3 for Critical Care Medicine the Ministry Health, First Center 300192, Affiliated Hospital Hebei Baoding 071000, These authors have contributed equally to this work Correspondence to: Wang, email: lailwq@126.com wangjinhuanfch@126.com Keywords: miR-433-3p, CREB, glioma, carcinogenesis, chemosensitivity Received: June 16, 2016     Accepted: November 22, Published: December 03, 2016 ABSTRACT Previous studies reported that miR-433 exerts function widely in human tumorigenesis development. Here, we further investigate potential role glioma. Quantitative real-time PCR demonstrated miR-433-3p miR-433-5p were low expressed glioma tissues cell lines. Functional suggested overexpression suppressed proliferation, induced apoptosis inhibited invasion migration cells. But growth metastasis cells not significantly influenced by miR-433-5p. In a xenograft model, also showed had an inhibitory effect on Bioinformatics coupled with luciferase western blot assays revealed CREB is direct target can rescue phenotype changes overexpression. Besides, could increase temozolomide targeting vitro vivo . Taken together, these results suggest may as marker diagnostic therapeutic