Effect of isocaloric low-fat diet on human LAPC-4 prostate cancer xenografts in severe combined immunodeficient mice and the insulin-like growth factor axis.

摘要: Over-consumption of dietary fat has been suggested to promote the development and progression prostate cancer in men. The present study was conducted answer following questions: ( a ) Can reduction decrease tumor growth rates Los Angeles (LAPC)-4 xenografts severe combined immunodeficient (SCID) mice independent total caloric intake? b Is insulin-like factor (IGF) axis involved effects on LAPC-4 SCID mice? Twenty-eight male CB17 beige (8 weeks old) were individually caged, randomized, fed an isocaloric high-fat (HF, 42% kcal) or low-fat (LF, 12% diet. Each mouse s.c. injected with 1 × 10 5 cells, volumes measured weekly. At week 16, all animals sacrificed, serum tumors obtained for analysis. Although intakes weights equal between groups, LF had significantly slower lower prostate-specific antigen levels compared HF mice. insulin, IGF-1 mRNA expression, IGFBP-2 immunostaining higher IGFBP-1 (by Western ligand blot) relative There no differences IGFBP-3 IGFBP-4 groups. cells cultured vitro media containing from demonstrated than serum. These results demonstrate that intake diet associated diet, intake, this effect may be mediated through modulation insulin/IGF axis.